Use this hub when the organism is anaerobic, acid-fast, intracellular, nonculturable, cell wall-deficient, or too thin/fastidious for routine Gram stain and plate logic.
Escalate MTB, Brucella, Francisella, Coxiella, and suspicious high-risk patterns before extra manipulation.
Aspirate, tissue, respiratory sediment, lesion swab, blood, serum, urine, and CSF answer different questions.
Choose NAAT, serology, special culture, direct microscopy, or reference testing before routine biochemical panels.
Specimen quality, transport, oxygen tolerance, reduced media, selective plates, and morphology-first workups.
Organism groups coveredBacteroides, Prevotella / Porphyromonas, Fusobacterium, Clostridium-like rods, Anaerobic cocci
Acid-fast organisms, tuberculosis safety logic, mycobacterial culture, NAAT, Runyon grouping, and NTM clues.
Organism groups coveredMTB complex, M. avium complex, M. kansasii, M. marinum, Rapid growers
Culture-negative syndromes where NAAT, serology, tissue stains, paired sera, and exposure history drive testing.
Organism groups coveredChlamydia, Rickettsia / Orientia, Ehrlichia / Anaplasma, Coxiella, Tropheryma
Cell wall-deficient bacteria with weak Gram stain value, special media, NAAT-first workflows, and beta-lactam resistance.
Organism groups coveredM. pneumoniae, M. genitalium, M. hominis, Ureaplasma urealyticum / parvum
Thin helical bacteria where syndrome timing, darkfield, serology, NAAT, and exposure history matter most.
Organism groups coveredTreponema, Borrelia burgdorferi, Relapsing fever Borrelia, Leptospira
